gel shift

SAM b7jp at
Thu Sep 5 03:53:10 EST 1996

I'm having problems with the competition assay on the gel shift i.e. the
cold oligo does not block or even decrease the binding of the hot one
when incubated with a rat liver nuclear protein extract. However, the
bands are clear and nice and on the same level as the HeLa cell control
(competition assay works for the comercial HeLa extract).  Did anyone
have a similar problem and/or has any ideas on what could cause it?
Thank you.
Anna Liachenko 
Mcgill University, Montreal, Canada

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