Expression vector with lacIq
spenneb at uni-muenster.de
Fri Sep 6 05:09:23 EST 1996
In article <322DDE1E.7E96 at szn1.nott.ac.uk>, Phil Hill
<sczpjh at szn1.nott.ac.uk> wrote:
> Hi there
> anyone know of a souce for an expression vector
> with lac promoter, NdeI or NcoI site to overlap start codon and lacIq on
> it....I need to shut down expression of a toxic protein in salmonella
> til I need it !
Try out the pTRCHis (A-C) from Invitrogen. They have got all you want and
additionally a His-Tag. The His-Tag is removed by cloning in the Nde I
site in case you don't like it.
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