Vector for direct cloning of PCR products

Torben Gjetting tor at biobase.dk
Thu Sep 5 17:23:01 EST 1996


jlight at SICKKIDS.ON.CA wrote:
> 
> Other than the Invitrogen TA cloning kit, are there other vectors available
> for direct cloning of PCR products?  Thanks in advance.  Jeff.

You can make your own cloning vector: 
Incubate linearized blunt-ended vector with dTTP and taq at 72C for 10 
min. 

Good luck.
Torben Gjetting



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