silent restriction map

Julian Parkhill J.Parkhill at bham.ac.uk
Mon Sep 9 05:32:33 EST 1996


In article <GaryTruett.1192474683A at PostOffice.PBRC.edu>,
pntrue at unix1.sncc.lsu.edu wrote:

> The software GCG can identify potential restriction sites through its
> mismatch function.
> The command line:
> map mis=1 filename
> directs the software to list restriction sites that would be created by
> making a single base substitution in the target sequence.

or, even better, use:

map (or mapsort) -silent

which will show all the restriction sites which can be generated (by any
number of changes) without altering the translation product, if you
specify the start of the gene.

-- 
          Julian Parkhill   -   J.Parkhill at bham.ac.uk
   CRC Laboratories, Inst. of Cancer Studies, Medical School,
 University of Birmingham, Edgbaston, Birmingham, B15 2TJ, U.K.
Disclaimer: If I thought anybody gave a damn about my opinions....



More information about the Methods mailing list