Synthetic Gene

Joe Maa mbi.tech at IX.NETCOM.COM
Mon Sep 9 17:03:18 EST 1996


Rafa made a good point.

I also have difficult to make more than 6 oligo-overlap PCR work.
So in your case, you may try PCR two smaller fragment first, then PCR
whole things!  make sure to sequence cloned products.
I have encounted all kinds of deletion/insertion!

good luck


quinn
http://www.netcom.com/~mbi.tech/



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