Genomic Library Construction

John Watson watson_j at bms.com
Mon Sep 9 09:19:34 EST 1996


Michael W. Thompson wrote:
> 
> I wish to construct a human genomic library from EcoRI digested genomic DNA (the
> fragment I'm looking for is 9.2 kb according to the southern blot) and was 
> wondering if anyone out there had experience with this and could suggest a vector 
> system or strategy.  I considered lambda bluestar (from Novagen), as this vector 
> has EcoRI sites, but they onlysell the uncut DNA in 10ug packages (hardly enough 
> from what I understand).  Does anyone out there know of a vector that could be 
> used or an alternative strategy?  Any help would be appreciated

It sounds like what you want to do is build a Mini-library of size-selected Eco-RI 
digested material in the, say 8.5 - 10.5 kb size range.  IMHO, you could use any 
plasmid vector for fragments that size and screen using colony lift or similar 
technique.
----------------
John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
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