Transfection of HeLa cells

Agustin Alconada alconada at embl-heidelberg.de
Tue Sep 10 12:37:39 EST 1996


Hello everyone,

   Does anybody know how the different promoters found in common
eukaryotic expression vectors compare in terms of expression levels in
HeLa cells? For example, should I expect higher expression levels of my
protein if I transfect the HeLa cells with a SV40, a SFFV- or a
CMV-promoter based vector?

Thanks



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