Long Repeat Cloning

stanyon at wehi.edu.au stanyon at wehi.edu.au
Tue Sep 10 01:21:06 EST 1996


HI,
	I'm looking for some advice on the cloning of repetative sequences. I
have a 1.5kb fragment which I can obtain with either EcoRI-XhoI or BamHI-BglII
sticky ends, the sequence of which is essentially a 27bp repeat (unique in the
databases so far . . .!). 
	Has anyone ever cloned such a piece of DNA and dismissed it as an
artefact, or managed to subclone something similar; I get clones, but they are
all odd sizes, and have extra restriction sites as well.

Thanks in advance

CAS 




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