Transfection of HeLa cells

jstrassw at OPAL.TUFTS.EDU jstrassw at OPAL.TUFTS.EDU
Wed Sep 11 09:35:08 EST 1996


Hello!
I can not say which is better because other factors (splice sasette in 
plasmid, 5' and 5' ujntranslated sequences) definately affect 
expression.  We use CMV-based plasmids for expression in HeLa, but our 
transfection comtrol plasmid is SV40 promoter/enhancer B-gal construct.  
I would like to know the answer to your question too.

Good Luck,
John


------------------------------------------------------------------------
John Strasswimmer, MD,PhD Candidate    | Phone (617) 636 8396
Tufts - New England Medical Center     | Fax   (617) 636 6190
Box 166                                | Email:  jstrassw at opal.tufts.edu
Boston, MA 02111 USA                   |                              
     HTTP://WWW.healthsci.tufts.edu/microbiology/strass/JSpage.HTML
     *** Co-Author of "HyperBug" Microbiology Teaching Software ***
                                       |
Remember, Your employer is eavesdropping...  
------------------------------------------------------------------------

On Tue, 10 Sep 1996, Agustin Alconada wrote:

> Hello everyone,
> 
>    Does anybody know how the different promoters found in common
> eukaryotic expression vectors compare in terms of expression levels in
> HeLa cells? For example, should I expect higher expression levels of my
> protein if I transfect the HeLa cells with a SV40, a SFFV- or a
> CMV-promoter based vector?
> 
> Thanks
> 
> 



More information about the Methods mailing list