Sticky-blunt ligations

Steven Cohen sbc4 at PO.CWRU.EDU
Wed Sep 11 20:41:55 EST 1996

At 06:17 PM 9/11/96 GMT, you wrote:
>I'm having similar problems. Couldn't figure out why. 
>But a note on your specific problem - Sma I is notorious for not doing
>the job right and giving a nice blunt end. You can of course easily 
>get around your porblem by using Xma I (same site as Sma I, but leaving
>an overhang).
>Boston University

I have not heard of this problem with Sma. We use it routinely in our lab.
On the other hand, attempts to use Xma have yielded very poor results. The
enzyme is typically sold in low u/ul and has been an extremely slow and
inefficient enzyme in our hands.


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