Electroporation condition for Jurkat cells
h-j.schaefers at popHost.com
Thu Sep 12 18:31:12 EST 1996
Alan Lau wrote:
> I use an electroporation method almost exactly the same as this and it
> works reliably. However, I cannot get electroporation efficiencies
> greater than around 15-20% of the total surviving cells.
> Does anyone have any comments, suggestions or alternative methods (e.g.
> lipofection) to improve this efficiency?
I whish i had such a great efficiency by transfecting rat mesangial cells via
electroporation. The max. i got is 1.5 to 2 % !!!
And this was 1000 fold higher than using lipofectin (DOTAP)!!
So i think you don't have to worry about your excellent 15-20% :)
Dipl. Biol. Heinz-Juergen Schaefers
University of Erlangen, Med. IV, Nephrology Research Lab
Loschgestr. 8, D-91054 Erlangen, Germany +49 (0)9131 85-
E-mail: h-j.schaefers at popHost.com -9206 (voice) -9202 (fax)
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