Silver Staining and BME

Jeff Cohlberg cohlberg at csulb.edu
Fri Sep 13 13:21:39 EST 1996


Achim Recktenwald (achim at ibex.ca) wrote:
: zoltan at bcmp.med.harvard.edu wrote:
: > 
: > I'm looking for a good solution to an old problem - those nasty
: > "pseudo-bands" in the 70kDa range which can plague silver-stained
: > protein gels.  I recently finished off a bottle of beta-mercaptoethanol
etc etc etc

It may be useful to know that these bands are skin keratins, probably
derived from contact of fingers with the solutions or glassware.  See
Ochs, D. (1983) "Protein contaminants of sodium dodecyl
sulfate-polyacrylamide gels," Analytical Biochemistry 135, 470-474

Jeff Cohlberg	California State University, Long Beach



More information about the Methods mailing list