antibodies to histag proteins

Malini Rajagopalan malini at uthct.edu
Fri Sep 13 14:19:21 EST 1996


Hi,

I am planning to make antibodies to histag proteins without cleaving 
the histidine tag. However, I am concerned about getting more 
background in western blots and immunoprecipitations using antibodies 
made to uncleaved histag proteins. Does anyone have any experience in 
this matter ?

Malini Madiraju



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