PCR of a single copy gene...

Taek H. You tyou at postbox.acs.ohio-state.edu
Sun Sep 15 01:06:13 EST 1996


In article <323A49D2.33DE at netvigator.com> lumdicks at NETVIGATOR.COM (Dickens Lum) writes:


>Dear,

>     I'm now trying to obtain a single copy gene via PCR. However, as
>large copies of pseudogenes exist in the genome, the PCR products are
>all come from those pseudogenes and not the functional one. Do anyone
>know how to deal with it..??? Any information and discussionn are
>greatly appreciated. Thanks.

>Regards,
>John Lum

Would RT-PCR work for the purpose?
Since only the functional gene makes a message, you don't have to worry about 
any products from pseudogenes unless you contaminate RNA with genomic DNA.




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