PCR of a single copy gene...

Michelle Gleeson michelle at MOLECULE.BIO.UTS.EDU.AU
Sun Sep 15 18:29:17 EST 1996



On Sun, 15 Sep 1996, Taek H. You wrote:

> In article <323A49D2.33DE at netvigator.com> lumdicks at NETVIGATOR.COM (Dickens Lum) writes:
>
>
> >Dear,
>
> >     I'm now trying to obtain a single copy gene via PCR. However, as
> >large copies of pseudogenes exist in the genome, the PCR products are
> >all come from those pseudogenes and not the functional one. Do anyone
> >know how to deal with it..??? Any information and discussionn are
> >greatly appreciated. Thanks.
>
> >Regards,
> >John Lum
>
> Would RT-PCR work for the purpose?
> Since only the functional gene makes a message, you don't have to worry about
> any products from pseudogenes unless you contaminate RNA with genomic DNA.
>
>
I would recommend Promega's RQI RNAse free DNAse to treat your RNA preps,
it worked really well for me with RTPCR
No affiliations, etc
Good luck,
Michelle
michelle.gleeson at uts.edu.au>




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