Does anyone have any references on the stability of dNTP?

Barry Mook barry at sccs.swarthmore.edu
Mon Sep 16 09:54:36 EST 1996


Hi,

   I'm a novice at lab techniques and have a question regarding dNTP
stability too.  How many times can you thaw and refreeze an aliquot of
dNTP/TE without reducing effectiveness.  How long can you leave an aliquot
at room temp before it's not worth refreezing?

Thanks,

Barry


In article <50urvl$i83 at sparcserver.lrz-muenchen.de>,
diabetes at lrz.uni-muenchen.de wrote:

> Hi. I have been using dNTPs dissolved in TE and frozen at -20C when
> not in use for several month without any problems in PCR.
> You could ask suppliers like Pharmacia for stability data.
> 
> Wolfgang
> Wolfgang Schechinger
> Institute for Diabetes Research
> Kölner Platz 1
> 80804 Muenchen
> Germany
> Phone +49 (89) 30 79 31 24
> Fax            30 81 73 3
> E-mail: DIABETES at LRZ.UNI-MUENCHEN.DE



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