Adipose tissue

[Eric Lader]

In article <elauzier-1209960856120001 at mac109.peps.ulaval.ca> Edouard
Lauzier, elauzier at fse.ulaval.ca writes:
>Ever tried to extract RNA from adipose tissue with samples of 50 mg or less ?
>
> If so could you give me some insights on your protocol or technique ??? 
>Because I'm trying and it doesn't yield any RNA at all !!  I'm using
>Sacchi (1987)

Adipose is mostly fat (no news there). Try some additional Chloroform
extractions to lose the lipids. 

               **my opinions are just that**
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        Eric Lader Ph.D.	Senior R&D Scientist,  
                          Ambion Inc.
Unique tools for Molecular Biology Research

Phone (800) 888-8804       Fax (512) 445-7139
				  (512) 445-6979       lader at ambion.com
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