RT_PCR: Oligo d(T) vs. Hexamers

Eric Lader lader at ambion.com
Mon Sep 16 21:51:01 EST 1996


In article
<c=US%a=_%p=MedImmune._Inc.%l=MEDIMMUNE_5-960916195241Z-2026 at medimmune-
.medimmune.com> Walsh, WalshB at MEDIMMUNE.COM writes:
>Is there an advantage to using random hexamers rather than  oligo d(T)
>when doing the first strand synthesis reaction of an RT-PCR? Is it any
>help when looking for rare transcripts?

target specific phenomenon. Try both.


               **my opinions are just that**
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        Eric Lader Ph.D.	Senior R&D Scientist,  
                          Ambion Inc.
Unique tools for Molecular Biology Research

Phone (800) 888-8804       Fax (512) 445-7139
				  (512) 445-6979       lader at ambion.com
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