Spin-X gel extraction problems

Barry Mook barry at sccs.swarthmore.edu
Mon Sep 16 13:50:48 EST 1996


Hi,

   I'm a lab newbie and am having a little trouble with some basic cloning
protocols.  I am currently using a costar filter centrifuge unit (SPIN X)
to gel purify my insert before doing a blunt end ligation with BM ligase. 
Has anyone had problems using Spin X columns to purify DNA?

Barry Mook
barry at sccs.swarthmore.edu

PS: I'll be QIA'ing next, but I was curious to see if others had run into
similar problems.



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