mRNA isolation method using Invitrogen spin columns

Eric Lader lader at ambion.com
Tue Sep 17 21:50:03 EST 1996


>> Does anyone have a good protocol to isolate mRNA from total RNA using
>> spin columns like Invitrogen are selling? I'm looking for an
>> inexpensive way to get mRNA from the Total RNA I already have isolated
>> from various rat and rabbit tissues. I called Invitrogen today and all
               



You can probably do a batch dT right in the guanidinium lysis buffer and
do your washes on a small spin cloumn with a frit on the bottom. Don't
tell anyone I told you this :-)

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        Eric Lader Ph.D.	Senior R&D Scientist,  
                          Ambion Inc.
Unique tools for Molecular Biology Research

Phone (800) 888-8804       Fax (512) 445-7139
				  (512) 445-6979       lader at ambion.com
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