Growth of H. pylori?
rand777 at ids.net
Tue Sep 17 07:14:25 EST 1996
On Mon, 16 Sep 1996 17:51:34 GMT, weber at cmb.UNR.EDU (Lee Weber) wrote:
>Does anyone have a robust method for growing H. pylori? We need to grow
>enough to isolate at least 100 ug of DNA. So far, we have had little success
>growing the stock from ATTC on blood agar in a candle jar. Is there a way to
>grow them in liquid culture or at least in the absence of potential
>contamination with heterologous (blood) DNA.
>Thanks in advance for any help at all
One can use campy-thio enriched with nad, cocarboxylase, B12 and
hemin...if the hemin is a problem, it can be left out....better growth
if medium contains hemin. If you want to try this I will send you the
formula via e-mail. The prepared medium is available from Randolph
Biomedical - 401-826-1407 - rand777 at ids.net---. Robert Randolph
I am employeed by Randolph Biomedical.
rrandy at hotmail.com
More information about the Methods