direct sequencing (35S manual) from PCR product?

Jeremy Marcus marcusj at
Tue Sep 17 15:19:06 EST 1996

I'm searching for a protocol which would allow me to sequence directly
from a pcr product of around 450bp.  The Sequenase protocol has been
recommended as useful for direct sequencing in general, but I'm using
standard 35S manual sequencing, and wonder if the Sequenase system can be
adapted for this application.  Any suggestions/protocols would be
Thanks!  Yvonne Barnes
email: barnes at

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