Does anyone have any references on the stability of dNTP?

brett brett at BORCIM.WUSTL.EDU
Tue Sep 17 19:33:35 EST 1996


>On 16 Sep 1996, Barry Mook wrote:
>
>> Hi,
>> 
>>    I'm a novice at lab techniques and have a question regarding dNTP
>> stability too.  How many times can you thaw and refreeze an aliquot of
>> dNTP/TE without reducing effectiveness.  How long can you leave an aliquot
>> at room temp before it's not worth refreezing?
>> 
>
>        I've had an dNTP mix lose effectiveness after only a handful of 
>cycles.  But lately, I've been using dNTPs from Pharmacia and I've 
>noticed no loss of effectivness after about 20 cycles.  If you're not 
>sure if dNTPs at room temp are any good, you can always try them in a 
>reaction and see what you get.
>
>-Frank
>fwilkins at thunder.ocis.temple.edu

I'd check the pH of your stocks, Frank, and adjust accordingly. There should
be no problem otherwise.


Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

"I own my own pet virus. I get to pet and name her." - Cobain




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