Does anyone have any references on the stability of dNTP?
brett at BORCIM.WUSTL.EDU
Tue Sep 17 19:33:35 EST 1996
>On 16 Sep 1996, Barry Mook wrote:
>> I'm a novice at lab techniques and have a question regarding dNTP
>> stability too. How many times can you thaw and refreeze an aliquot of
>> dNTP/TE without reducing effectiveness. How long can you leave an aliquot
>> at room temp before it's not worth refreezing?
> I've had an dNTP mix lose effectiveness after only a handful of
>cycles. But lately, I've been using dNTPs from Pharmacia and I've
>noticed no loss of effectivness after about 20 cycles. If you're not
>sure if dNTPs at room temp are any good, you can always try them in a
>reaction and see what you get.
>fwilkins at thunder.ocis.temple.edu
I'd check the pH of your stocks, Frank, and adjust accordingly. There should
be no problem otherwise.
Program in Immunology
Washington University - St Louis
brett at borcim.wustl.edu
"I own my own pet virus. I get to pet and name her." - Cobain
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