Reverse Northern?

Jeff "Newt" Sekelsky fznewt at peseta.ucdavis.edu
Wed Sep 18 11:27:43 EST 1996


On Tue, 17 Sep 1996, Chihara wrote:

> I have a paper with a reference to a Reverse Northern, what is that?
> Thanks
> 
> 

A reverse northern is a method for finding transcribed sequences within a
cloned genomic region.  The genomic clones are on the filter.  The probe
is made by reverse transcribing from RNA (usually mRNA).  The probe is
then a complex mixture of first strand cDNA, but because the target
genomic DNA on the filter is in such abundance, you can get enough
hybridization to see which bands contain transcribed regions.




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