PCR reaction (polymerase mixtures)
tysondr at sluvca.slu.edu
Wed Sep 18 11:50:45 EST 1996
Loesch-Fries Lab wrote:
> I plan to do primer mediated mutagenesis using PCR.
> Is there anybody who use Vent polymerase for primer extension?
> If yes then have you used mixture of vent and Taq (from promega)?
> If yes then what and how did you mex each other?
> Does the mixture work well?
I use Pfu from Stratagene (when it's not backordered!) to minimize
mutations. It's worked for me.
Many companies are now selling mixed enzymes; check Gibco-BRL
(Life Technologies) and Promega.
Darren Tyson, Grad Student Cell and Molecular Biology
tysondr at sluvca.slu.edu or darren at primary.net
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