long pcr

Karl Gately gatelk at whio.lincoln.ac.nz
Fri Sep 20 09:56:44 EST 1996


In article <51q44h$jm2 at news.acns.nwu.edu> ageorge at casbah.acns.nwu.edu (ANNE GEORGE) writes:
>From: ageorge at casbah.acns.nwu.edu (ANNE GEORGE)
>Subject: long pcr
>Date: 19 Sep 1996 00:29:05 GMT


>I am trying to amplify sequences of 10kb or so from genomic DNA. Havent had
>any 
>luck so far. Used different types of kits but no use at all. Is there any 
>solution.
>Thanks-- 
>ANNE GEORGE
>Northwestern University, Evanston, IL.   USA
>ageorge at casbah.acns.nwu.edu


Hi Anne,
Have you tried Expand High Fidelity Polymerase??  I am successfully 
amplifying a 2kbp fragment with it (with a massive yield) and it can 
supposedly amplify vastly in excess of this.

Regards,
Karl




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