Tet-regulation

Brendan barry at sccs.swarthmore.edu
Thu Sep 19 10:06:49 EST 1996


Hi,

   Is anyone using the Tet-inducible system of Bujard?  I'm having lower
inductions that his lab suggests (1000-10,000-fold).  Using the
Luc-reporter that they provide I'm getting a mere 20 fold or so after
creating stable rtTA's in HeLa....any ideas?

Barry



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