Phage Display of cytosolic Protein
tomweber at bway.net
Thu Sep 19 08:02:48 EST 1996
I want to display a cytosolic protein, and thereafter random mutants
of it, as a fusion protein with a protein of a filamentous phage, much
like petide libraries.
Here my questions:
has anyone of you out there ever done this and what was your
experience with the nonreducing environment that the usually cytosolic
proteins are now exposed to ?
What is the best vector (phagemid) to use and where can I get it from
Did any of you ever use the expression module of the Pharmacia
Recombinant Phage display Antibody System (RPAS) for a similar
purpose, and how are your experiences ?
Thanks for your input
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