best kit for cloning PCR products?

Dr. Peter Gegenheimer pgegen at kuhub.cc.ukans.edu
Fri Sep 20 19:18:10 EST 1996


In <3239A8BF.1640 at lilly.com>, Michele Dowless <mdowless at lilly.com> writes:
>Jeff Cohlberg wrote:
> Any recommendations as
>> to the easiest way to clone PCR products?  Any particularly efficient
>> kits?

The simplest way is to use a proof-reading polymerase, such as VENT (N.E.B.), Pfu (Stratagene), or Pwo (B.M.B), which generate blunt-ended products, and clone into the blunt-end site (eg. SmaI) of any commercial vector. Save $ for a really rainy day.

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