Amp stock survival?
trevor.fenning at hri.ac.uk
Fri Sep 20 03:47:17 EST 1996
Not long is the answer - ampicillin goes off very fast indeed (half
life at 37oC = 18 hours) even without resistant bacteria degrading it,
so don't kep stocks at room temp, or even in the fridge.
I keep 1000x stocks made up in 1 ml aliquots at -20 which seem to
last, although they will deterioate eventually. If you are using it
to select for transformants, always include controls of
non-resistant/non transformed cells on the amp media. That way you
will notice any loss of effectiveness early.
Trevor Fenning, HRI - Wellesbourne.
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Subject: Amp stock survival?
Author: server-daemon <server-daemon at dl.ac.uk> at INTERNET-MAIL
Date: 9/19/96 3:18 PM
How long can you leave an Amp stock 100X at room temp before you should
toss it? Can you tell I made a boo-boo?
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