in cell RT-PCR

David N. Levy levy at uab.edu
Fri Sep 20 14:15:56 EST 1996


In article <51ote8$ifm at pulp.ucs.ualberta.ca>
aszczepe at gpu5.srv.ualberta.ca (Agnieszka Szczepek) writes:

> Any working protocols for in cell RT-PCR (in solution NOT in situ)?
>                                           ^^^^^^^^^^^
> I would appreciate any help,
> 
> Thanks,
> 
> Agnieszka


Do you mean in situ RT-PCR on cells in suspension as opposed to cells
bound to a slide?

See:
GJ Nuovo, PCR Methods and Applications, 1995, 4:S151-S167
Patterson et al., Science 1993, 260:976


David N. Levy
Department of Medicine
University of Alababa, Birmingham
Birmingham, AL 35294

levy at uab.edu



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