help for ligation

Qunfeng Dong qfdong at iastate.edu
Fri Sep 20 13:56:09 EST 1996


Hi, sorry if this topic has been discussed before. One of my friend asked me
what's best ratio between insert and vector. His insert is about 900 bps and
his vector is about 12 kb, Both are cut by EcorI. Anyone experienced with
large vector ligation before? and what's the DNA amount in ligation?

Thanks a lot.

-- 
Qunfeng Dong
qfdong at iastate.edu



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