GST-fusion protein

Hiroshi Ohnishi oni at
Sat Sep 21 20:44:48 EST 1996

I have been trying to purify a GST-fusion protein on the Pharmacia 
sepharose coloumn. My protein does not elute from the coloumn 
effectively (less than 5%).
I would like to purify the protein as active form.

Any suggestions?


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