<Construction of a targeting vector>

phil coffino coffino at cgl.ucsf.edu
Mon Sep 23 14:06:02 EST 1996


Hi 

I' m trying to make a targeting vector for knock out manipulations and 
I have some problems for my last step of subcloning. I have already 
cloned two genomic fragments of 2 and 5 kb in a pGEM7zf+ vector, and 
for the moment i did not succeed in the last cloning off the 5 kb 
selection cassette.  It's a cloning in the Xho I site. 
Is there a particular way for cloning in a large plasmid (10 kb) ?

Thanks



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