Help! Direction of ligation

Irina A. Libova ialibova at ARTSCI.WUSTL.EDU
Mon Sep 23 18:40:59 EST 1996


Hi!
I am ligating an insert into a vector, using HindiIII site on both 
ends.  And I need a specific orientation.  But the problem is that on 
ALL of my clones I get the reverse orientation compared to the one I 
need, instead of getting a random 50:50 distribution of orientations.  
Has anybode had the same problem, and what can I do to change the 
orientation?



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