Help! Direction of ligation

[Irina A. Libova]

Hi!
I am ligating an insert into a vector, using HindiIII site on both 
ends.  And I need a specific orientation.  But the problem is that on 
ALL of my clones I get the reverse orientation compared to the one I 
need, instead of getting a random 50:50 distribution of orientations.  
Has anybode had the same problem, and what can I do to change the 
orientation?