precipitating from Qiaquick

:-Peter obrien at pharm.med.upenn.edu
Sun Sep 22 12:08:00 EST 1996



@--> In article <51u40m$hp9 at paperboy.ccc.nottingham.ac.uk>,
@--> pdxrmb at vaxb.ccc.nottingham.ac.uk wrote:
@--> 
@--> # I have been using Qiagens qiaquick columns for the purification of PCR
@--> products and bands form gels. Direct elution yields good quality DNA, but
@--> the concentration is a little low - I would prefer a nice high
@--> concentration stock for storage etc. Ethanol precipitation produces very
@--> low yields (as judged from EtBr gels)despite there being plenty of DNA in
@--> the eluate- is there a reason for this ?
@--> # I thought maybe carryover of chaotropic salt may effect the
@--> precipitation conditions ? but wasnt exactly ur why this shoud be. any
@--> advice/infromation would be gratefully received. thanks in advance-
@--> Richard M Badge.


Try eluting with warm dH2O or TE.

:-Peter








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