RT-PCR and RBC lysis (rather than ficoll)

Administrador de iihema.fmed.uba.ar postmast at iihema.fmed.uba.ar
Wed Sep 25 19:50:56 EST 1996




>I have no personnal experience of this but, yes, I heard a couple of 
>times that hemoglobin was a very effective PCR inhibitor. Maybe in your 
>case the ficoll separation is also useful as an enrichment into tumor 
>cells.

I read somewhere that what actually inhibits the PCR is the high concentration
of iron released from lised red blood cells.


Facundo Garcia Bournissen.
postmast at iihema.fmed.uba.ar



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