RT-PCR and RBC lysis (rather than ficoll)

Administrador de iihema.fmed.uba.ar postmast at iihema.fmed.uba.ar
Wed Sep 25 19:50:56 EST 1996

>I have no personnal experience of this but, yes, I heard a couple of 
>times that hemoglobin was a very effective PCR inhibitor. Maybe in your 
>case the ficoll separation is also useful as an enrichment into tumor 

I read somewhere that what actually inhibits the PCR is the high concentration
of iron released from lised red blood cells.

Facundo Garcia Bournissen.
postmast at iihema.fmed.uba.ar

More information about the Methods mailing list