Mold on yeast plates
colin at fungus.biochem.ualberta.ca
Wed Sep 25 21:28:59 EST 1996
In article <52cca4$e09 at nntp3.u.washington.edu>, yacman at homer12 (L.
> Greetings Earthlings,
> I'm trying out the yeast 2-hybrid system, but I'm relatively
> inexperienced with yeast. After yeast transformation I am incubating the
> yeast at 30 degrees in a dry incubator for about 8-10 days.
> Unfortunately, I have a pesky mold problem. Any suggestions about how I
> might prevent this without killing the yeast?
> Thank you,
You can't since they are both fungi. The only way to deal with this is
through good aseptic technique. I would restreak the yeast strains out to
single colonies to make sure they aren't infected. In addition, I would
make sure the plates are clean to begin with. Put them in the incubator
for a few days to see if anything grows. Finally, if you are storing your
plates in a cold room prior to use, DON'T. Cold rooms are notorious for
Hope this helps.
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