Wanted - Fly cDNA - Yeast e

David_Sutherland at CBRC.MGH.HARVARD.EDU David_Sutherland at CBRC.MGH.HARVARD.EDU
Wed Sep 25 08:03:01 EST 1996


                       Subject:                               Time:3:03 PM
  OFFICE MEMO          Wanted - Fly cDNA - Yeast expression_  Date:9/22/96
Hi

This is a rather specific request - does anybody out there have a
Drosophila cDNA library (any stage,  although embryonic may be preferable)
in a yeast expression vector which they feel free to share?   The library
needs to have the following specifications

(i) It must not be fused to an activation domain (like standard 2 hybrid
libraries)

(ii) Expression must not be under the control of a Gal4 requiring promoter
(eg:- Gal10)

(iii) Selectable marker should not be Ura3.

I would also be interested to know if anyone has a randomed primed fly cDNA
library suitable for a yeast-2-hybrid/interaction trap screen.

Thanks in advance
David Sutherland






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