RNA extraction from PBMC

tilahun jiffar Tilahun.Jiffar at utmb.edu
Thu Sep 26 11:11:45 EST 1996


I separate PBMC from heparinized human whole blood using 
Ficoll-hypaque, wash it once, and isolate RNA using Trizol. My RNA yield has 
been consistently low (about 1ug/10 6 cells). I tried every possible 
optimization method. Didn't work. If you have similar experiences and 
possible solution, please let me know.



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