Gibco Exon Trap System - pSPL3 vector & N blot backgrnd

Lab jzhang at biovax.rutgers.edu
Thu Sep 26 17:32:01 EST 1996


Hi all,

  Using this system, the final (secondary) PCR product contains a couple 
of hundred bases of vector sequences along with the "trapped" 
exon-sequence.  This type of product was used as template for 
random-primed radio-labeling (without first removing the flanking pSPL3 
vector sequences) for use as a Northern blot probe.
  Has anyone had a problem with the pSPL3 vector sequences themselves 
picking up things on a Northern blot - thus confusing the results?

Thanks.



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