HybSpeed RPA Kit

Shelley Cole shelley at DARWIN.SFBR.ORG
Thu Sep 26 20:25:24 EST 1996


I had similar trouble with their kit.  The first thing that I did was to 
make sure that my labeled probe and sample RNA was EXTREMELY clean before 
the first precipitation.  Then, I included a 70% ETOH wash of my final 
pellet before resuspending in gel loading buffer.

Hope this helps.
Shelley


On 25 Sep 1996, Harold Rees wrote:

> Hi 
> I have been working with the HybSpeed RPA kit from  Ambion, but I have 
> had significant problems with redissolving the RNA in both the 
> hybridization buffer and the gel loading buffer.  The RNA usually gets 
> stuck in the wells of my gel and creates a huge mess.  Anyone have any 
> suggestions?  I have tried using more buffer, but that hasn't worked.  
> 
> Thanks.
> 
> --
> Hal Rees                                ********************************
> Monell Chemical Senses Center           *"Oh well, whatever, nevermind"*
> Philadelphia, PA                        *  -Kurt Cobain                * 
> hrees at mail.sas.upenn.edu                ********************************
> 
> 



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