GST-fusion protein

crs0580 at inforamp.net crs0580 at inforamp.net
Fri Sep 27 23:04:44 EST 1996


Hiroshi Ohnishi wrote:
> 
> I have been trying to purify a GST-fusion protein on the Pharmacia
> sepharose coloumn. My protein does not elute from the coloumn
> effectively (less than 5%).
> I would like to purify the protein as active form.
> 
> Any suggestions?
> 
> Thanks
> Oni
Ensure that the pH of your reduced glutathione solution is at least pH8
(i.e., not acidic).



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