GST-fusion protein

crs0580 at crs0580 at
Fri Sep 27 23:04:44 EST 1996

Hiroshi Ohnishi wrote:
> I have been trying to purify a GST-fusion protein on the Pharmacia
> sepharose coloumn. My protein does not elute from the coloumn
> effectively (less than 5%).
> I would like to purify the protein as active form.
> Any suggestions?
> Thanks
> Oni
Ensure that the pH of your reduced glutathione solution is at least pH8
(i.e., not acidic).

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