Protein cross-linking in crude bacterial cell extract.
nikolaic at VISAR.WUSTL.EDU
Fri Sep 27 17:23:17 EST 1996
> Date: Fri, 27 Sep 1996 16:25:58 -0400
Bin LIN <binlin at umich.edu> wrote:
> Hello netters:
> I am planning to indentify proteins, in crude bacterial cell extract,
> interacting with a bacterial G-protein by chemical cross-linking.
> As you might know, there is 0.1mM PMSF in the cell extract buffer. I
> am wondering whether PMSF will interact with cross-linker (DMA or
It should not.
> Could anyone give me some advice on setting isotonic extracting buffer
> and choosing appropriate cross-linkers? BTW, what's the best
> terminating buffer for cross-linking reaction?
1 M glycin, or 1M Tris. The x-linker works on primary amines.
There is such thing as PIERCE catalog. Look up there.
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