(no subject)

Chi-Kuang Wen cwen at aux.btny.purdue.edu
Sat Sep 28 01:37:40 EST 1996


Hongyu Yang wrote:
> 
> Dear netter:
> 
> I isolated DNA from plant leaves which was so viscous that we could not
> separate it with an agarose gel after "digestion". In a previous posting,
> I was told that the stuff which made the DNA solution viscous was
> polysachharides. I tried several methods to get rid of the
> "polysaccharide", but failed. The methods I tried included 10-30% ethanol
> precipitation, PEG-precipitation.  I'll greatly appreciate any advices.
> 
> Thanks
> 
> HY Yang
Hi, HY:

Try CTAB! CTAB binds to the nucleic acids at hight-salt conditions while
the CTAB-DNA/RNA complex precipitates at low-salt conditions. Using this 
property, most of the polysacchardies may be eliminated.

Chi-Kuang



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