Viscous DNA solution

Hongyu Yang yang at tifton.cpes.peachnet.edu
Sat Sep 28 19:25:17 EST 1996


Dear netter:

I isolated DNA from plant leaves which was so viscous that we could not
separate it with an agarose gel after "digestion". In a previous posting,
I was told that the stuff which made the DNA solution viscous was
polysachharides. I tried several methods to get rid of the
"polysaccharide", but failed. The methods I tried included 10-30% ethanol
precipitation, PEG-precipitation, and CTAB.  I'll greatly appreciate any 
advices.


Thanks

HY




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