ta-cloning vector

[dreamer at super.zippo.com]

xxxxx at biolan.uni-koeln.de (xxxxxxx) wrote:

>I´m not really happy with my home made TA-cloning vector. May somebody mail
>me a protocoll working in her/his hands focused on yield of plasmid,TAQ units,
>T4 Ligase units and duration and temperature for the religation to 
>separate vector with from vector without an overhang. 
>thanks in advance
>Michael

>Please respond to my Email adress
>MKroiher at novell.biolan.uni-koeln.de

Check the following paper in the January 96 issue of BioTechniques. It
is an improved method for making your own TA-cloning vector:

Preparation of T-overhang vectors with high PCR product cloning
efficiency
-Hadjeb, N. and Berkowitz, G.A.
BioTechniques 20(1), January 1996

Hope this helps.