ta-cloning vector

dreamer at super.zippo.com dreamer at super.zippo.com
Sat Sep 28 12:56:30 EST 1996

xxxxx at biolan.uni-koeln.de (xxxxxxx) wrote:

>I´m not really happy with my home made TA-cloning vector. May somebody mail
>me a protocoll working in her/his hands focused on yield of plasmid,TAQ units,
>T4 Ligase units and duration and temperature for the religation to 
>separate vector with from vector without an overhang. 
>thanks in advance

>Please respond to my Email adress
>MKroiher at novell.biolan.uni-koeln.de

Check the following paper in the January 96 issue of BioTechniques. It
is an improved method for making your own TA-cloning vector:

Preparation of T-overhang vectors with high PCR product cloning
-Hadjeb, N. and Berkowitz, G.A.
BioTechniques 20(1), January 1996

Hope this helps.

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