Electroporation of lymphoid and myeloid cell lines

Susan suecam at WELCHLINK.WELCH.JHU.EDU
Mon Sep 30 16:22:09 EST 1996


Has anyone out there had any luck electroporating the following cell
lines with a BioRad machiine:

U-937

KG1a

A3.01 (a subclone from CEM, I believe)

HL-60

I've had success with HEL cells and DAMI cells, but all of these lines
sem to be a little bit different, and lipofectamine (Gibco BRL) doesn't
work at all (at least with KG1a and U-937 cells)

Let me know at : ecameron at welchlink.welch.jhu.edu



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