GST-fusion protein breakdown

gc genecutl at mendel.nospam.berkeley.edu
Tue Apr 1 21:40:16 EST 1997


In article <marcvdc.160.06C54F02 at lmb1.rug.ac.be>, marcvdc at lmb1.rug.ac.be wrote:

* Our GST-fusion protein is well expressed in E. coli and it is soluble, but it 
* runs about 8kDa lower than expected on PAGE. I fear we have breakdown of the 
* fusion. 

   By "lower then expected", is that based on the predicted molecular weight?
   If so, it may be just that the protein runs anomalously in the gel.
   You could try using in vitro transcription/translation to synthesize
   some protein and run it on the same gel as the E. coli protein to see
   if there is a difference in size.

--gc



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