GST-fusion protein breakdown
genecutl at mendel.nospam.berkeley.edu
Tue Apr 1 21:40:16 EST 1997
In article <marcvdc.160.06C54F02 at lmb1.rug.ac.be>, marcvdc at lmb1.rug.ac.be wrote:
* Our GST-fusion protein is well expressed in E. coli and it is soluble, but it
* runs about 8kDa lower than expected on PAGE. I fear we have breakdown of the
By "lower then expected", is that based on the predicted molecular weight?
If so, it may be just that the protein runs anomalously in the gel.
You could try using in vitro transcription/translation to synthesize
some protein and run it on the same gel as the E. coli protein to see
if there is a difference in size.
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