MacConkey instead of XGAL ?

Greg Gloor ggloor at julian.uwo.ca
Tue Apr 1 13:13:03 EST 1997


> > Since MacConkey plates can distinguish lac+ from lac- colonies,
> > why not use MacConkey + amp plates for blue/white (in this case red/white)
> > selection?

This works fine for the combination of BluescriptII and the XLI blue
E.coli strain. Just make sure you incubate at 37 degrees and the
difference is very clear.



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